SERVICES AVAILABLE

A. Tube Plasmid Clones – Standard primers

1. Gel run only                                                             $ 3.00
2. Cost/reaction  (if we do both PCR and gel run)     $10.00
3. Colony Sequencing            (we do DNA prep)        $20.00

B. 96 Well Plates Plasmid Clones

1. Gel run only                                                     $100/ 96 well plate
2. Cost/ reaction                                                  $350/ 96 well plate, $6,650 for 20 plates.
3. Colony sequencing:                                           $500/plate, $9, 000 for 20 plates.

C. Tube BAC Clones End Sequence per end            in 96 wells                in tubes

1. Gel run only                                                              $ 2.00                          $2.00
2. Cost/reaction  (if we do both PCR and gel run)      $20.00                         $25.00
3. Colony Sequencing                                                   $30.00                         $35.00

D. BAC Subclone Sequencing

1. Library Cost: $500 per BAC if we generate the nebulized 2 Kbp and 10 Kbp insert libraries.
2. Colony sequencing: Our fees for SIUC users are $450/plate including plasmid preparation, $9, 000 for 20 plates.
3. For sequencing just plasmid DNA our fees are $350/ 96 well plate, $6,650 for 20 plates.
4. These fees do not include bioinformatics, but you will get PHRED scores and success rates /plate. 
5. Bioinformatics fees which include trimming off vector sequences, BLAST for bacterial chromosomal DNA, RNA, viral RNA, rRNA and mitochondrial DNA are $1.30/sequence.
6. Therefore, a finished 8.9 fold redundant BAC sequence for a BAC plus vector of 135Kbp will cost $11,954 based on about 1,920 sequence reads ($9,000 + $500 +  $2,496). For BAC plus vector of 100kbp it would be $7,972.40 based on 1,248 reads, 7.1 fold redundant ($5,850 + $500 + $1,622.40) (see Table 1).
7. Annotation. We will not do this. Try the  Genquire package, info can be found at : http://bioinfo.pbi.nrc.ca/

E. Run your own gels on our machines.

1. Gel run only              $ 0.50 per sample minimum of 40 provide own plates ($500)
                    

Machines.

1.PE377 automated DNA sequencer.

2. CEQ2000 fully automated capillary DNA gel caster, loader and sequencer.