Center for Electron
Microscopy Center for Electron
Microscopy
The Center for Electron Microscopy (CEM) at Southern Illinois University is a service unit supported by the Office of Research Development and Administration (ORDA). The CEM provides service and training in electron and light microscopy. This is accomplished by means of graduate level courses in scanning (SEM) and transmission electron microscopy (TEM) as well as training in the use of all instrumentation in the CEM. Trained staff will prepare specimens and assist in the viewing of samples and assist in the writing of technical or budgetary proposals related to electron microscopy and the writing of reports for granting agencies. CEM sponsors workshops on modern research methods in electron microscopy and offers tours of the facility to the university and community.
The internal rates are designed to recover all costs associated with expendible supplies, service contracts on major instrumentation and a portion of the salaries of technical personnel.
The CEM is available for outside grant and contract work. Outside rates are negotiated through the Office of Research Development and Administration and are designed to recover all operating expenses as well as an established overhead rate.
Major equipment consists of a state-of-the-art analytical system composed of a Hitachi 7100 TEM equipped for x-ray analysis (NORAN Voyager III) and digital, high resolution imaging at 125 kV. The TEM is connected to a variable pressure SEM also with x-ray analysis capability. This permits researchers to observe non-conductive specimens under more natural conditions than the conventional SEM and TEM (also available). The EM system is networked with various light microscopes and macroscopes capable of capturing low light level images for further analysis. Preparatory equipment includes: ultramicrotomes, vacuum evaporators, critical point and freeze dryers, sputter coater, specimen trimmers, darkroom set-ups, copy stands, and miscellaneous small lab equipment.
Electron Microscopes $15.00/hr X-ray Analysis 20.00/hr Light Microscopes 10.00/hr Image Analysis 10.00/hr
Critical Point Drying 15.00 Freeze Drying 20.00 Slam Freezing 20.00 Sputter Coating 10.00 Vacuum Evaporation 20.00 Ultramicrotomes (per hr) 10.00
Electron Microscopes $25.00/hr X-ray Analysis 25.00/hr Light Microscopes 20.00/hr Image Analysis 20.00/hr
Pricing varies depending upon amount of processing needed. Biological specimens, which require more steps than non- biologicals, will be more costly. A precise figure will be provided after determination of specific needs. Cost will range from $20.00 to over $100.00. Savings may be realized if some processing is done by the investigator and completed by the CEM.
Transmission EM (up to 5 spec/ vial) $40.00 Ultramicrotomy (routine, 3 grids) 20.00 Grid staining (Ur + Pb, 3 grids) 15.00 Scanning EM (biologicals) 40.00 Scanning EM (non-biologicals) 20.00 Critical Point Drying 20.00 Freeze Drying 20.00 Slam Freezing 20.00 Sputter Coating 10.00 Vacuum Evaporation 25.00
Other SIU Services