PREPARATION OF DNA TEMPLATES;

                 Suggested Purification Methods with QIAGEN kits:

Plasmid DNA           QIAGEN Spin mini kit#(27104 (50 prep) or#27106 (250 prep)
                                   QIAGEN Plasmid Mini kit #12123 (25 preps)

PCR Product            QIAGEN Gel Extraction Kit #28704 (50 preps)

BAC DNA methods have  been optimized but are only 70-80 % efficient.

Alkalyne  lysis protocols and training can be provided on request.

QIAGEN products are available at the automated DNA sequencing facility. See Rubina Ahsan for a product list and save shipping and handling and 5% discount.

The ABI kits are available from applied Biosystems (800) 327-3002.

This will determine the cycle sequencing program used to incorporate the fluorescent dideoxynucleotides into extension products. Each kit basically comes with Taq DNA polymerase, fluorescent-ddNTPs, & dNTPs. There are four different fluorescent dyes that distinguish between the four bases in DNA. The reaction is linear, only one primer is used per reaction.

The CEQ8000 kits are available from Beckman. The dyes are infrared and so cause lesser steric inhibition of chain extension.

Soybean Genomics Center remains open from 8AM to 5PM five days a week.