siu1.jpg (4081 bytes) dna1.jpg (3330 bytes)Plant Biotechnology and Genome Centerdna1.jpg (3330 bytes)

 

 

 

Two plant transformation ready bacterial artificial chromosome libraries for soybean: 
Applications in chromosome walking and genoe wide physical mapping. (MS Word version)
 

 

Meksem K. Zobrist K, Ruben E, Hyten D, Quanzhou T, Zhang H.B. and D.A. Lightfoot. 2000. Two transformation ready large insert clone libraries for soybean: Physical mapping of resistance to Soybean Cyst Nematode and Sudden Death Syndrome. Theor. Appl. Genet. 101:747-755.
  Coyne, C., Meksem, K. Lightfoot, DA., Keller KE, Martin RR, McClendon MT, Inglis DA, Storlie EW, and McPhee KE. 2000. Construction of a bacterial artificial chromosome library for pea (Pisum sativum L.). Pisum Genetics 32:23-26
 

 

Men A, Meksem K., Kassem MA, Lothar, D., Stiller J, Lightfoot DA, Gresshoff P.M. 2001.bacterial Artificial Chromosome Library of Lotus japonicus constructed in an Agrobacterium tumefaciens- transformable vector.
Molecular Plant Microbe Interactions 14:422-425.
 

Rajesh P.N., Meksem K., Coyne C.J., Lightfoot D.A., and Muehlbauer F.J. 2002. Construction of the first bacterial artificial chromosome library in chickpea. International Chickpea and Pigeonpea Newsletter 9:29-30.

 

Chang YL, Tao Q, Scheuring C, Ding K, Meksem K, Zhang HB. An integrated map of Arabidopsis thaliana for functional analysis of its genome sequence.Genetics. 2001 Nov;159(3):1231-42.
  Rajesh PN, Coyne C, Meksem K, Sharma KD, Gupta V, Muehlbauer FJ.Construction of a HindIII Bacterial Artificial Chromosome library and its use in identification of clones associated with disease resistance in chickpea.

 

Wu C , Sun S, Nimmakayala P , Santos FA, Springman R , Ding K , Meksem K , Lightfoot DA and Zhang HB. 2004. A BAC and BIBAC-based Physical Map of the Soybean Genome. Genome Research 14:319-326.

 

Common Name
Cultivar
 Species Enzyme Plants
Vector
Soybean Forrest Glycine max BamHI pCLD04541(V41)
Soybean Forrest Glycine max HindIII pCLD04541(V41)
Thale Cress Columbia Arabidopsis thaliana BamHI pCLD04541(V41)
Thale Cress Columbia Arabidopsis thaliana HindIII pCLD04541(V41)
Potato   Solanum tuberosum BamHI pCLD04541(V41)
Barley   Hordeum vulgarae HindIII pCLD04541(V41)
Pea 269818 Pisum sativum HindIII pCLD04541(V41)
Chickpea FLIP 84-92C Cicer arietinum HindIII pCLD04541(V41)
Chickpea FLIP 84-92C Cicer arietinum BamHI pCLD04541(V41)
Chickpea FLIP 84-92C Cicer arietinum EcoRI pCLD04541(V41)
Japanese Trefoil Gifu Lotus japonicus HindIII pCLD04541(V41)
Wild soybean PI Glycine soja HindIII pCLD04541(V41)
Wild soybean PI Glycine soja BamHI pIndigoBAC5
Tree moss   Physcomitrella patens HindIII pCLD04541(V41)
Tree moss   Physcomitrella patens BAmHI pCLD04541(V41)
Chile pepper   Capiscum annuum HIndIII pIndigoBAC5
Chile pepper   Capiscum annuum BamHI pIndigoBAC5
Sugar Beet   Beta Vulgaris HindIII pIndigoBAC5
Glycine polploid PI 441001 Glycine tomentella BamHI pIndigoBAC5
Strawberry diploid   Fragaria vesca BamHI pIndigoBAC5
Strawberry octaploid Senga Sengana Fragaria ananassa BamHI pIndigoBAC5
Arabidopsis species Enquire Arabidopsis sp. HindIII pIndigoBAC5