Biofilm Sampling Device
Project Leaders: Mohamed El-Azizi and Nancy Khardori
Unit: School of Medicine, Springfield
Brief Description:
Biofilms consist of microorganisms attached to a surface through an extracellular polymeric network, composed mainly of polysaccharides. The microorganisms within the biofilm are extremely difficult to detach and are also greatly resistant to treatment compared to free microorganisms. The biofilm sampling device allows for modeling of biofilm formation and testing of possible treatments. This technology consists of simple tubing fitted within a larger tubular chamber. Fluid flow can be directed and controlled through the chamber to simulate static conditions and various in-vivo conditions. The device allows for simulation of numerous medical devices, including catheters and stents. Once the biofilm forms on the device, it may be removed for direct study. Additionally, the device can be used to model real-time conditions to determine the efficacy of biofilm treatments on the formed biofilm. Finally, the device may be pre-treated with biofilm-fighting agents to investigate biofilm prevention.
Patent Status:
Patent pending.
Potential Commercial Uses:
Increased resistance by biofilms to treatment creates a problem for indwelling medical devices, especially those used for a relatively long time period (30 days to 12 or more months). Biofilms can cause infection, sepsis, or even death. The device provides adaptability for modeling various conditions, and further versatility for testing biofilm prevention and treatment methods. There are few alternative biofilm testing devices currently available, none of which effectively mimic in-vivo conditions.
Contact:
Rob Patino, SIU School of Medicine - Office of Technology Transfer, (217) 545-8167, fax: (217) 545-0786