Conservation genetics applies genetic methods to handle conservation questions such as the maintenance, loss, and restoration of biodiversity. Genetic questions regarding phylogenetic classification, levels of genetic variation, and population genetic structure can all be considered components of conservation genetics. Many species conservation management plans can be improved by the input of genetic information; the bobcat (Lynx rufus) is one such species, due to its habitat being increasingly fragmented and past exploitation as a furbearer. To date, little is known about the genetic status of the bobcat. Using both nuclear and mitochondrial DNA markers, I will examine the population structure of the bobcat at different spatial scales. First, I will assess relatedness among bobcat groups and between bobcat individuals in southern Illinois. Second, I will estimate levels of genetic diversity, and connectivity among bobcat populations in Illinois, Missouri, Indiana, and Kentucky. Lastly, I will re-examine the sub-specific designations assigned to the bobcat. The information provided by these genetic analyses would help determine priorities for bobcat management and help assess where gaps in knowledge may exist.